Anti Phospholipid Antibody ELISA- The only assay detecting antibodies to all 8 phospholipid antigens coated

 Antiphospholipid syndrome (APS) is an autoimmune disease with various clinical manifestations, including arterial and venous thrombosis, thrombocytopenia, recurrent fetal loss and livedo reticularis as well as cardiac, hematological and neurological manifestations. In its worst manifestation, APS presents as the so-called catastrophic APS with disseminated thrombus formation in a variety of vessels leading to multi-organ failure and thrombocytopenia.

APS is caused due to Antiphospholipid antibodies which comprises of a heterogenous group of  autoantibodies primarily described as antibodies directed against various negatively charged phospholipids.

Two major serological parameters for diagnosis, anticardiolipin and anti-ß 2-GPI antibodies, have been discovered quite some time ago and are well established. There is, however, increasing evidence from a number of studies that testing a profile of various phospholipids apart from cardiolipin and ß 2-GPI increases both the sensitivity and specificity of the laboratory diagnosis.

Looking at the general composition of human cell membranes based upon six phospholipids it appeared obvious to check for autoantibodies against these, particularly as the distribution of these phospholipids strongly differs within cell compartments and cell types. Despite some contradictory results, a number of studies indicates that there is a sub-group of APS patients with a clear clinical picture of APS but without detectable cardiolipin / ß 2 -GPI antibodies or LA. However, other phospholipid antibodies can be detected in the serum of these patients, proving phospholipid profile and screening tests to be an important additional diagnostic tool.

Aesku Phospholipid Assays take care that all the antibodies against all the phospholipid antigens are detected and thus the wells are coated with all known phospholipid antigens viz. bovine cardiolipin + beta 2 GPI, phosphatidyl-serine, -inositol, -ethanolamine, -choline and sphingomyelin as compared to the assays available in the market.

Aesku offers the most comprehensive panel of phospholipid tests, employing highly purified,

Native human beta 2 GPI and bovine phospholipids.

Phospholipid screening tests are highly sensitive for a rapid and reliable combined determination of the most important antiphospholipid antibodies.

Phospholipid Profiles employing 8 different antigens, are the ideal tools for the reliable differential diagnosis of APS as certain antibody patterns can be correlated with distinct clinical manifestations of the APS.