Diaspot Neptune ANA 25 screen MultiQuant kit is composed of 24 nitrocellulose strips on which 25 antigens have been coated in a microdot-shaped format. The test is based on the simple format of ELISA wherein the intensity of the coloration on the strip is directly proportional to the amount of antibody present in the sample. All the measured results are fully quantitative thanks to a 6 point built-in calibration curve, including blank control. The strips are fully automated on Neptune instrument and the quantitative results are given by the Neptune quantitative software. Different types of controls (sample, conjugate and substrate) are also coated on the strips. Their presence validates the whole process of the test (from sample loading to substrate kinetics, through conjugate specificity / reactivity). For optimum precision, all dots are coated in a triplicate microdot format, allowing calculation of a mean value and a confidence interval for each parameter (antigens, calibration curve and controls).