Diaspot Neptune ANA 25 Multiquant


Different types of controls (sample, conjugate and substrate) are also coated on the strips. Their presence validates the whole process of the test (from sample loading to substrate kinetics, through conjugate specificity / reactivity). 
For optimum precision, all dots are coated in a triplicate microdot format, allowing calculation of a mean value and a confidence interval for each parameter (antigens, calibration curve and controls).

Diaspot Neptune ANA 25 screen MultiQuant kit is composed of 24 nitrocellulose strips on which 25 antigens have been coated in a microdot-shaped format. The test is based on the simple format of ELISA wherein the intensity of the coloration on the strip is directly proportional to the amount of antibody present in the sample.

All the measured results are fully quantitative thanks to a 6 point built-in calibration curve, including blank control. The strips are fully automated on Neptune instrument and the quantitative results are given by the Neptune quantitative software.

  • Upto 25 autoantibodies detected on a single strip.

  • Ideal screening tool for ANA alongwith Myositis(PM/DM), Sharp syndrome, Scelroderma, PBC and AIH on a single strip in a single run for a single patient. Thus cost saving.No need to purchase separate kits for Myositis, PBC and AIH.

  • Results obtained in 1 h with the Neptune Instrument.

  • The DIASpot Neptune kits, adapted for automation:

  • Strips are mounted on a special plastic support

  • Quantitative results and validation of the test with the Neptune Quantification Software.

  • Antigen and control in triplicates to ensure quality.

  • Reagents are provided in ready-to-use individual cartridges, to fit the Neptune automated instrument.

  • Complete integrated Calibration curve for full quantitative results.

  • Multiple built-in controls for validation of the test.

  • Reactive control or Positive control: validity of the test.

  • Conjugate control: control of the conjugate used.

  • Substrate kinetic: validity of the substrate.

  • 6 point calibration curve: quantitative interpretation.

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Automate ANA 25 multiquant with NEPTUNE

Neptune is a compact and innovative machine which processes the strips with ready-to-use reagents provided in individual cartridges 

Neptune Quantification Software:

  • Allows an easy semi-quantitative or quantitative interpretation. It only requires a computer and the Neptune scanner.

  • Scanner provided is a flat-bed scanner dedicated to the interpretation of blot strips. The scanner contains an internal standard scale which allows the calibration of the scanner at any time.

  • The interpretation software contains a database that keeps record of all saved results (numeric values and scanned images). So, one specific result can be found via a multi-criteria search engine.

  • The software can import and export data in CSV (Comma Separated Values) format from and to the Laboratory Information System (LIS). Final results can also be sent by e-mail (Outlook or Outlook Express), printed and saved on pdf format.

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